Advantages Of Serial Dilution-agar Plate
The main difference between pour plate and spread plate is that the molten is poured on to the inoculum during the preparation of the pour plate whereas inoculum is spread on the surface of the solidified agar during the preparation of the spread plate. Inoculum refers to microorganisms, bacteria or fungi that grow in or on the nutrient agar.Pour plate and spread plate are two techniques that quantify bacterial samples. Both require Petri dishes. Generally, pour plates is the method for counting the number of colony-forming bacteria present in a liquid specimen.
What Are Some Advantages Of Being Able To Create Dilutions
Pour plates also allow the identification of bacteria as, anaerobes or aerobes. On the other hand, spread plates allow the isolation of specific clonal colonies.
Key Areas Covered1.– Definition, Method, Importance2.– Definition, Method, Importance3.– Outline of Common Features4.– Comparison of Key DifferencesKey Terms: Colony counting, Colony Isolation, Nutrient Agar, Petri Dish, Pour Plate, Spread PlateWhat is a Pour PlatePour plate refers to a plate prepared by mixing the inoculum with cooled but, still molten medium before pouring the latter into the Petri dish. It is the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. A fixed amount of inoculum from sample is placed in the center of a sterile Petri dish and then the cooled, molten agar is poured on to the dish and mixed well.
Dilution Plate Method
The plate can be inverted and incubated after solidification.In the pour plate method, bacteria grow both on the surface as well as within the medium. Small colonies appear within the medium due to lack of oxygen.
One can count each colony in the plate as each colony represents a colony-forming unit (CFU). What is Spread PlateSpread plate is a technique that counts or isolates bacterial colonies on the surface of the agar. A small amount of inoculum can be poured on to the solidified agar and spread with the use of a spreader. Here, the spreader should not be too hot, as it may kill the bacteria in the sample. The bacterial colonies prepared by the pour plate technique is shown in figure 1.